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Applikon Micro-Flask

Cultivo reproducible en placas de microtitulación

Una plataforma tecnológica rentable y fácil de usar para el rápido manejo y crecimiento de un gran número de cepas microbianas, bibliotecas clonales, bancos de mutantes y líneas celulares. Este sistema permite que una sola persona cultive y pruebe miles de cepas simultáneamente con un mínimo de manipulaciones repetitivas.

Contactar con nosotros para más información

Your interest in bioreactor systems

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Argentina

AP Biotech
Phone: +541142921611
Email: info@apbiotech.com.ar
URL: www.apbiotech.com.ar

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Argentina

AP Biotech
Phone: +541142921611
Email: info@apbiotech.com.ar
URL: www.apbiotech.com.ar

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Brazil

Sotelab
+55 (21) 3392-3563
www.sotelab.com.br

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Brazil

Sotelab
+55 (21) 3392-3563
www.sotelab.com.br

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Chile, Peru

AP Bioprocess
www.ap-bioprocess.com

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Chile, Peru

AP Bioprocess
www.ap-bioprocess.com

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Colombia

Intek Group
Phone: +57 316 3550400 - 749 8437
Email: contacto@intekgroup.com.co
URL: www.intekgroup.com.co

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Colombia

Intek Group
Phone: +57 316 3550400 - 749 8437
Email: contacto@intekgroup.com.co
URL: www.intekgroup.com.co

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Czech Republic

Vekamaf
Phone: +420 284 690 881
URL: www.vekamaf.cz

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Czech Republic

Vekamaf
Phone: +420 284 690 881
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Denmark

Holm & Halby
Phone: +454326 9400
Email: info@holm-halby.dk
URL: www.holm-halby.dk

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Denmark

Holm & Halby
Phone: +454326 9400
Email: info@holm-halby.dk
URL: www.holm-halby.dk

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Estonia

Bio Techa
Phone: +372 6597101
Email: info@biotecha.ee
URL: www.biotecha.lt

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Estonia

Bio Techa
Phone: +372 6597101
Email: info@biotecha.ee
URL: www.biotecha.lt

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Hungary

Holimex
Phone: +36 1 391 4400
URL: www.holimex.hu

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Hungary

Holimex
Phone: +36 1 391 4400
URL: www.holimex.hu

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India

Spinco Biotech Pvt. Ltd.
Phone: +91 44 45678900
Email: info@spincotech.com
URL: www.spincotech.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

India

Spinco Biotech Pvt. Ltd.
Phone: +91 44 45678900
Email: info@spincotech.com
URL: www.spincotech.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Indonesia

PT Arico Sainsindo Nusantara
Phone: +62-21 580 6342 / 3
Email: arico@dnet.net.id
URL: www.ptarico.com

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Indonesia

PT Arico Sainsindo Nusantara
Phone: +62-21 580 6342 / 3
Email: arico@dnet.net.id
URL: www.ptarico.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Ireland

Asistec
Phone: +353(0)1286 8796
Email: info@asistec.ie
URL: www.asistec.ie

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Ireland

Asistec
Phone: +353(0)1286 8796
Email: info@asistec.ie
URL: www.asistec.ie

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Israel

Medent Group
Phone: +97286237984
Email: info@medent.co.il
URL: medent.co.il

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Israel

Medent Group
Phone: +97286237984
Email: info@medent.co.il
URL: medent.co.il

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Italy

Steroglass
Phone: +39 075 609091
Email: info@steroglass.it 
URL: www.steroglass.it

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Italy

Steroglass
Phone: +39 075 609091
Email: info@steroglass.it 
URL: www.steroglass.it

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Japan

Sanyo Trading Co., LTD
Phone: +81-3-3518-1196
URL: www.sanyo-trading.co.jp

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Japan

Sanyo Trading Co., LTD
Phone: +81-3-3518-1196
URL: www.sanyo-trading.co.jp

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Lithuania

Bio Techa
Phone: +370 5 237 60 07
Email: info@biotecha.lt
URL: www.biotecha.lt

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Lithuania

Bio Techa
Phone: +370 5 237 60 07
Email: info@biotecha.lt
URL: www.biotecha.lt

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Norway

Bio Nordica
Phone: 23 03 58 00
Email: info@bionordika.no
URL: bionordika.no

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Norway

Bio Nordica
Phone: 23 03 58 00
Email: info@bionordika.no
URL: bionordika.no

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Poland

Labo Baza
Phone: +4861 812 57 45
Email: biuro@labobaza.pl
URL: www.labobaza.pl

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Poland

Labo Baza
Phone: +4861 812 57 45
Email: biuro@labobaza.pl
URL: www.labobaza.pl

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Portugal

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

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Portugal

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Russia

Abtek
Phone: +7(495) 723-64-97
Email: abtek@abtek.ru
URL: abtek.ru

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Russia

Abtek
Phone: +7(495) 723-64-97
Email: abtek@abtek.ru
URL: abtek.ru

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Singapore

Zil Sci Pte. Ltd.
Phone: +65 6767 2929
Email: ENQUIRY@ZILSCI.COM
URL: www.zilsci.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Singapore

Zil Sci Pte. Ltd.
Phone: +65 6767 2929
Email: ENQUIRY@ZILSCI.COM
URL: www.zilsci.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Slovakia

Vekamaf
Phone: +421-2-52932891
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Slovakia

Vekamaf
Phone: +421-2-52932891
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Africa

Seperations
Phone: +27 11 919 1000
Email: info@separations.co.za
URL: separations.co.za

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Africa

Seperations
Phone: +27 11 919 1000
Email: info@separations.co.za
URL: separations.co.za

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Korea

SeouLin Bioscience
Phone: +821670-5911
Email: support@seoulin.co.kr
URL: www.seoulin.co.kr

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Korea

SeouLin Bioscience
Phone: +821670-5911
Email: support@seoulin.co.kr
URL: www.seoulin.co.kr

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Spain

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Spain

Aplitech Biolab
Phone: +34 629818802
www.aplitechbiolab.com

 

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Switzerland

Resea Biotec
Phone: +41 34 531 08 20
Email: office@reseabiotec.ch
URL: www.reseabiotec.ch

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Switzerland

Resea Biotec
Phone: +41 34 531 08 20
Email: office@reseabiotec.ch
URL: www.reseabiotec.ch

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Taiwan

Tseng Hsiang Life Science Ltd.
Phone: +862-27851156
Email: info@thco.com.tw
URL: www.thco.com.tw

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Taiwan

Tseng Hsiang Life Science Ltd.
Phone: +862-27851156
Email: info@thco.com.tw
URL: www.thco.com.tw

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Meditop Co., Ltd

Holm & Halby
Phone: +662 9331133
Email: info@meditopthailand.com
URL: meditopthailand.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Meditop Co., Ltd

Holm & Halby
Phone: +662 9331133
Email: info@meditopthailand.com
URL: meditopthailand.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Turkey

Ant Teknik
Phone: +90 216 422 6700
Email: antteknik@antteknik.com 
URL: www.antteknik.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Turkey

Ant Teknik
Phone: +90 216 422 6700
Email: antteknik@antteknik.com 
URL: www.antteknik.com

The Applikon Micro-Flask with various configurations of cover clamps and sandwich covers
The Applikon Micro-Flask by Duetz design

Acelere su proceso cultivando cepas en paralelo

  • Tasas de evaporación bajas y uniformes
  • Barrera estéril para alojamientos individuales que evita la contaminación cruzada
  • Velocidades de transferencia de oxígeno similares a las de los matraces de agitación en agitadores orbitales estándar
  • Conversión de placas de 24 y 96 microtituladores (placas profundas y de alojamiento bajo) en microbiorreactores individuales
  • Muestreo simultáneo y reproducible de 96 existencias de glicerol congelado con nuestro replicador criogénico

Toda la manipulación con el Micro-Flask se realiza en paralelo con conjuntos de 24 o 96 cepas. Proporciona un uso más eficiente de su agitador orbital actual, con un ahorro de tiempo de hasta 10 posibles. Permite la misma calidad de crecimiento que en los matraces Erlenmeyer en términos de tasas de transferencia de oxígeno y reproducibilidad.

Productos relacionados

Microrreactores individuales

El Micro-Flask convierte las placas de microtitulación de 6, 24 y 96 alojamientos en microrreactores individuales, lo que permite realizar cultivos simultáneos y reproducibles.

Transferencia de oxígeno

La transferencia de oxígeno en el Micro-Flask es similar a la de los fermentadores, lo que permite un cultivo preciso.

Especificaciones técnicas

Tipo de
placa de microtitulación
Volumen de alojamiento Volumen de cultivo Frecuencia de agitación orbital Amplitud de agitación Tasa de transferencia de O2
(30
 ˚C, aire, 1 bar)
Tasa de refresco de la cámara de aire Tasa de evaporación por alojamiento
(a 30
 ˚C)
Patrón de mezcla
a 300
 rpm
             

 

ampl.
25 mm
ampl.
50 mm
Polipropileno
de alojamiento profundo de 24 cuadrados,
17 × 17 mm,
profundidad de 40 mm
11 000 µL 2500 µL 300 rpm 50 mm 51 mmol O2/l/h 2,5 ml/min
(1 VVM)

50 % de humedad:
50 µL H2O al día

75 % de humedad:
25 µL H2O al día

2500 µL 2500 µL
2500 µL 300 rpm 25 mm 39 mmol O2/l/h
2500 µL 220 rpm 50 mm 35 mmol O2/l/h
         
4000 µL 300 rpm 50 mm 24 mmol O2/l/h 2,5 ml/min
(0,6 VVM)
4000 µL 220 rpm 25 mm 24 mmol O2/l/h
Poliestireno
de alojamiento de 24 círculos, ⌀16 mm,
profundidad de 18 mm
3000 µL 750 µL 300 rpm 50 mm 40 mmol O2/l/h 1,1 ml/min
(1,4 VVM)

50 % de humedad:
30 µL H2O al día

75 % de humedad:
15 µL H2O al día

1000 µL 1000 µL
750 µL 300 rpm 25 mm 25 mmol O2/l/h
         
1000 µL 300 rpm 50 mm 30 mmol O2/l/h 1,1 ml/min
(1,1 VVM)
1000 µL 300 rpm 25 mm 19 mmol O2/l/h
Polipropileno
profundo de alojamiento de 96 cuadrados,
8 × 8 mm,
profundidad de 40 mm
2400 µL 500 µL 300 rpm 50 mm 38 mmol O2/l/h 1 ml/min
(2 VVM)

50 % de humedad:
22 µL H2O al día

75 % de humedad:
11 µL H2O al día

750 µL 750 µL
500 µL 300 rpm 25 mm 12 mmol O2/l/h
         
750 µL 300 rpm 50 mm 24 mmol O2/l/h 1 ml/min
(1,3 VVM)
750 µL 300 rpm 25 mm 7 mmol O2/l/h
         
1000 µL 300 rpm 50 mm 18 mmol O2/l/h 1 ml/min
(1 VVM)
1000 µL 300 rpm 25 mm 3 mmol O2/l/h
poliestireno
de alojamiento bajo de 96 círculos,
⌀6,5 mm,
profundidad de 11 mm
380 µL 100 µL 300 rpm 50 mm 39 mmol O2/l/h 250 µL/min
(2,5 VVM)

50 % de humedad:
6 µL H2O al día

75 % de humedad:
3 µL H2O al día

150 µL 150 µL
100 µL 300 rpm 25 mm 20 mmol O2/l/h
         
150 µL 300 rpm 50 mm 32 mmol O2/l/h 250 µL/min
(1,7 VVM)
150 µL 300 rpm 25 mm 16 mmol O2/l/h
         
200 µL 220 rpm 50 mm 12 mmol O2/l/h 250 µL/min
(1,3 VVM)
200 µL 300 rpm 25 mm 12 mmol O2/l/h