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Applikon BioSep

Scalable cell culture perfusion

The Applikon BioSep is a unique cell retention device for the process of constant feeding of fresh media and removal of spent media, while retaining a high number of viable cells. It uses high frequency resonant ultrasonic waves to separate cells and thereby automatically removes cell debris, which makes it the perfect solution for continuous bioprocesses.

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Your interest in bioreactor systems

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Argentina

AP Biotech
Phone: +541142921611
Email: info@apbiotech.com.ar
URL: www.apbiotech.com.ar

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Brazil

Sotelab
+55 (21) 3392-3563
www.sotelab.com.br

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Chile, Peru

AP Bioprocess
www.ap-bioprocess.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

China

Applitech
Phone: +862034821111
Email: biotech@applitechpharma.com 
URL: www.applitechpharma.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Colombia

Intek Group
Phone: +57 316 3550400 - 749 8437
Email: contacto@intekgroup.com.co
URL: www.intekgroup.com.co

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Czech Republic

Vekamaf
Phone: +420 284 690 881
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Denmark

Holm & Halby
Phone: +454326 9400
Email: info@holm-halby.dk
URL: www.holm-halby.dk

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Estonia

Bio Techa
Phone: +372 6597101
Email: info@biotecha.ee
URL: www.biotecha.lt

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Hungary

Holimex
Phone: +36 1 391 4400
URL: www.holimex.hu

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

India

Spinco Biotech Pvt. Ltd.
Phone: +91 44 45678900
Email: info@spincotech.com
URL: www.spincotech.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Indonesia

PT Arico Sainsindo Nusantara
Phone: +62-21 580 6342 / 3
Email: arico@dnet.net.id
URL: www.ptarico.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.  

Ireland

Asistec
Phone: +353(0)1286 8796
Email: info@asistec.ie
URL: www.asistec.ie

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Israel

Medent Group
Phone: +97286237984
Email: info@medent.co.il
URL: medent.co.il

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Italy

Steroglass
Phone: +39 075 609091
Email: info@steroglass.it 
URL: www.steroglass.it

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Japan

Sanyo Trading Co., LTD
Phone: +81-3-3518-1196
URL: www.sanyo-trading.co.jp

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Lithuania

Bio Techa
Phone: +370 5 237 60 07
Email: info@biotecha.lt
URL: www.biotecha.lt

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Norway

Bio Nordica
Phone: 23 03 58 00
Email: info@bionordika.no
URL: bionordika.no

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Poland

Labo Baza
Phone: +4861 812 57 45
Email: biuro@labobaza.pl
URL: www.labobaza.pl

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Russia

Abtek
Phone: +7(495) 723-64-97
Email: abtek@abtek.ru
URL: abtek.ru

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Singapore

Zil Sci Pte. Ltd.
Phone: +65 6767 2929
Email: ENQUIRY@ZILSCI.COM
URL: www.zilsci.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Slovakia

Vekamaf
Phone: +421-2-52932891
URL: www.vekamaf.cz

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Africa

Seperations
Phone: +27 11 919 1000
Email: info@separations.co.za
URL: separations.co.za

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

South Korea

SeouLin Bioscience
Phone: +821670-5911
Email: support@seoulin.co.kr
URL: www.seoulin.co.kr

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Switzerland

Resea Biotec
Phone: +41 34 531 08 20
Email: office@reseabiotec.ch
URL: www.reseabiotec.ch

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Taiwan

Tseng Hsiang Life Science Ltd.
Phone: +862-27851156
Email: info@thco.com.tw
URL: www.thco.com.tw

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Meditop Co., Ltd

Holm & Halby
Phone: +662 9331133
Email: info@meditopthailand.com
URL: meditopthailand.com

We have a local distributor for this country. Feel free to reach out directly via below phone number, email or website for quickest response.

Turkey

Ant Teknik
Phone: +90 216 422 6700
Email: antteknik@antteknik.com 
URL: www.antteknik.com

The ideal solution for long-term cultivation

  • Automatic removal of cell debris
  • No damage to the cells
  • No fouling or blocking allows for long term operation
  • Scalable perfusion device (0,1 – 1.000 L / day)

High separation efficiency

Using the technology of acoustic resonance allows for high separation efficiency (99%) without damaging any cells. One standard mode of operation employs, for example, a harvest pump at the exit port of the resonator chamber, and a recirculation pump for the return of separated cells that settled from the acoustic energy field within the resonator chamber. Alternatively, the Applikon BioSep system can be set up to allow for semicontinuous operation or alternative strategies of cell recirculation.

Easy to install and operate

The Applikon BioSep can be mounted directly onto the bioreactor head plate. An extensive characterization has proven full scalability between the different sizes allowing you to start in perfusion mode directly at the beginning of your process optimization all the way through to production.
The Applikon BioSep system is available in 1 L/day, 10 L/day, 50 L/day, 200 L/day and 1000 L/day and is compatible with any brand of bioreactor.

Proven under cGMP conditions

The Applikon BioSep system is used as perfusion device in various commercially operated cGMP processes. The 50 L/day and 200 L/day separation model are by default supplied with all documentation and services required for cGMP operation.

Specifications

BioSep system 1L 10L 50L 200L 1000L
Maximum perfusion rate 1 L/day 7 L/day 45 L/day 200 L/day 1000 L/day
Minimum perfusion rate 0.1 L/day    0.7 L/day 4.5 L/day 20 L/day 100 L/day
Separation efficiency Up to 99% (depending on cell concentration and perfusion flow)
Minimum cell concentration 2 x 105 cells/ml (depending on cell size)
Bioreactor connection 6 mm OD tube 12 mm OD tube Mounted separately Mounted separately Mounted separately
Medium inlet 3 mm hosebarb 6 mm hosebarb 10 mm hosebarb 0.5” TC 0.5” TC
Return line to bioreactor 3 mm hosebarb 6 mm OD diptube 0.5” TC 0.5” TC 0.5” TC
Perfusion outlet 3 mm hosebarb 6 mm hosebarb 6 mm hosebarb 0.5” TC 0.5” TC
Weight 0.1 kg 0.5 kg 1.5 kg 13 kg 70 kg
Resonator volume 0.7 ml 7 ml 50 ml 290 ml 1450 ml
           
BioSep Controller APS 995 APS 990 APS 990 APS 992 APS 993
BioSep Controller Power 15 Watt 150 Watt 150 Watt 350 Watt 1500 Watt

BioSep Controller Dimensions

(DxWxH, mm)

Combined with my-Control 305x130x130 305x130x130 450x400x135 450x300x450
BioSep Controller Weight (kg) Combined with my-Control 3.5 3.5 17.6 43